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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
上海觅拓生物科技有限公司
- 规格:
30个/盒
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文献和实验) at the final concentration of 1 tablet/50ml): PBS Buffer A: 10 mM Hepes, pH 7.9, 10mM KCl, 1.5mM MgCl2, 0.5mM DTT S1 solution : 0.25 M Sucrose, 10 mM MgCl2 S2 solution: 0.35 M Sucrose, 0.5 mM MgCl2 S3 solution: 0.88 M Sucrose, 0.5 mM MgCl2
Growing Cells in Geltrex Reduced Growth Factor Basement Membrane Matrix
a sufficient amount of diluted Geltrex solution to cover the entire area onto growth surface (1.5 ml for 35 mm dish, 3 ml for 60 mm dish). 5) Coat the dish and place at room temperature for a minimum of 60 minutes. 6) For long term
The ribonuclease protection assay (RPA)
by gentle flicking and quick spin in a microfuge. Incubate at 37°C for 30 minutes. 3. Add the following reagents (in order) to each 1.5 ml Eppendorf tube: 26 µl 20 mM EDTA 25 µl Tris-saturated phenol 25 µl chloroform:isoamyl alcohol (50
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