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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Acetylcholinesterase (Ache)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Acetylcholinesterase (Ache)品名简称:
Recombinant Rat Ache protein货号:
CSB-EP001154RA规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
AcheUniprot No.:
P37136别名:
(AChE)种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
68.9 kDa表达区域:
32-614aa氨基酸序列:
EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGILDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLIWIYGGGFYSGASSLDVYDGRFLAQVEGTVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELISCLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPDALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPAHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTVEERIFAQRLMQYWTNFARTGDPNDPRDSKSPRWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.功能2:
/文献:
"Effect of acute noise stress on acetylcholinesterase activity in discrete areas of rat brain." Sembulingam K., Sembulingam P., Namasivayam A. Indian J Med Sci 57:487-492(2003)研究领域:
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文献和实验中乙酰胆碱酯酶(Acetylcholinesterase ,AChE) 活性明显降低[ 1 ] , 胆碱乙酰转移酶( Choline acetyltrans ferase ,ChAT) 活性下降[ 2 ] ,模型大鼠脑内乙酰胆碱(Acetylcholine ,ACh) 和多巴胺(Dopa amine , DA) 的含量均明显降低[ 3 ] ,说明向大鼠侧脑室持续灌注Aβ1~40能导致胆碱能和多巴胺能双重神经功能损伤。这与AD脑内胆碱能和单胺能功能障的病理表征相类似;还发现模型大鼠长时程
了大量的研究工作,但由于技术方法的限制,对神经递质的性质无法确定。1949年Koelle建立了胆碱酯酶染色法(cholinesterase staining method, ChE)1964Rh ,Karnovsky 和Roots在此基础上改良并建立了一步完成的乙酰胆碱酯酶(Acetycholinesterase,AChE)直接染色法,使显示胆碱能神经成为可能。从生物测定来看,AchE和胆碱能神经的标志酶存在平行关系。因此,一般认为,在加用乙酰胆碱酯酶抑制剂的情况下,用Ache 显示胆碱能神经
和Roots在此基础上改良并建立了一步完成的乙酰胆碱酯酶(Acetycholinesterase, AChE)直接染色法,使显示胆碱能神经成为可能。从生物测定来看,AchE和胆碱能神经的标志酶存在平行关系。因此,一般认为,在加用乙酰胆碱酯酶抑制剂的情况下,用AchE 显示胆碱能神经,仍不失为一种简便易行、可信的技术方法。但必须指出的是,一些接受胆碱能神经支配的细胞(称为胆碱能敏感细胞)和一些非神经细胞(如红细胞和骨骼肌)也可呈AchE阳性反应,甚至中枢神经系统内去甲肾上腺素(NA)能神经元集中的部位—蓝斑
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