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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Acetylcholinesterase (ACHE)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Acetylcholinesterase (ACHE)品名简称:
Recombinant Human ACHE protein货号:
CSB-MP001154HU规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ACHEUniprot No.:
P22303别名:
AChE种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Mammalian cell分子量:
66.0 kDa表达区域:
32-614aa氨基酸序列:
EGREDAELLVTVRGGRLRGIRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLVWIYGGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREAPGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCPPGGTGGNDTELVACLRTRPAQVLVNHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQVSDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLDPSRNYTAEEKIFAQRLMRYWANFARTGDPNEPRDPKAPQWPPYTAGAQQYVSLDLRPLEVRRGLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQDRCSDL蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.功能2:
/文献:
/研究领域:
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文献和实验开发一种靶点无关的光亲和标记方法,利用磺酰肼(sulfonylhydrazone)作为光亲和基团,在紫外光照射下生成活性卡宾,从而实现小分子配体与纯化单个蛋白的共价交联,并精确鉴定结合位点。实验以纯化的MAO-A、STAT5b、AChE和KRas G12D等蛋白为对象,将含有磺酰肼基团的配体或探针与蛋白在PBS缓冲液中孵育,随后在380 nm波长下照射约10分钟以引发卡宾生成并完成共价标记。标记后的蛋白经胰蛋白酶消化,采用LC-MS/MS进行肽段映射分析,通过二级质谱(MS/MS)碎片离子确认具体
Acetylcholinesterase (AChE) is the modulating enzyme of cholinergic systems. Study of its morphological distribution and its pathophysiological disposition following disease or exposure to anticholinesterase compounds such as pesticides
A Rapid Enzymatic Method for Aflatoxin B Detection
A novel method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE, from electric eel ) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman’s
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