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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated peptide derived from C-terminal of AGO3 protein of Chlamydomonas reinhardtii
- 形态:
Lyophilized
- 保存条件:
store lyophilized/reconstituted at -20°C; once rec
- 克隆性:
Polyclonal
- 标记物:
Chung et al. (2019) Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression. Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x.
- 适应物种:
Chlamydomonas reinhardtii
- 级别:
分子生物学级
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
1: 2500 (WB)
- 靶点:
Argonaute 3(AGO3) of Chlamydmonas reinhardii is cytoplasmic enriched and associated with polysomes and miRNAs (Chung et al. 2019).
- 抗体名:
AGO3-PAZ | Argonaute 3 PAZ domain (Chlamydomonas)
- 规格:
50 µg
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文献和实验made up of the amino-terminal, middle, and PIWI domains. The Piwi Argonaute Zwille (PAZ) domain is held above the base by a "stalk"-like region. The PIWI domain (named for the protein piwi) is similar to ribonuclease H, with a conserved active site aspartate-aspartate
域,它们是:1 个RNA 解旋酶―― DEXH 盒子、1 个含结合dsRNA 折叠的DUF283、1 个结合dsRNA 末端的PAZ、2 个RIII 和1 个dsRNA 结合结构域。PAZ 结构域同时存在于构成RNA 沉默复合物的Argonaute (Ago)蛋白质家族中,事实上,PAZ 就取名于3 个主要的Ago 蛋白质,即Piwi、Ago 和Zwille[12]。贾第虫Dicer 仅有1 个PAZ、2 个RIII 结构域[13],比哺乳动物Dicer 要小得多,但在体外却有正常的裁剪活性。对贾第虫Dicer 晶体
MicroRNAs and Messenger RNA Turnover
(4 ). AGO contains the conserved PAZ and PIWI domains, and seems to be the sole protein required for RISC-mediated activities (5). The discovery that the PIWI domain adopts a ribonuclease (RNase) H fold (6 –8 ) has led to a concept of AGO as an “mRNA
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