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- 文献和实验
- 技术资料
- 形态:
Immunogen affinity purified IgG.
- 保存条件:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
- 克隆性:
Polyclonal
- 供应商:
Agrisera
- 宿主:
Goat
- 靶点:
Goat anti-Human IgG (H&L), FITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum
- 抗体英文名:
Goat anti-Human IgG (H&L), FITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum
- 抗体名:
Goat anti-Human IgG (H&L), FITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum
- 规格:
2 mg
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Staining Procedure for Flow Cytometric Detection of Human Cyclins
unconjugated isotype controls and mAbs, add 20 m l of FITC-conjugated goat anti-mouse Ig (Cat. No. 12064D) at optimal working dilution. Incubate 30 min at RT in the dark. Add 2 ml Wash buffer, centrifuge 5 min at 200 x g Aspirate supernatant. 10
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