CpxA | Conjugative plasmid expression

CpxA | Conjugative plasmid exp

ression
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  • 询价
  • Agrisera
  • AS13 2653
  • 瑞典
  • 2025年11月23日
  • Western blot (WB)
  • Rabbit
  • Yersinia pseudotuberculosis
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    • 详细信息
    • 技术资料
    • 抗体名

      CpxA | Conjugative plasmid expression

    • 抗体英文名

      CpxA | Conjugative plasmid expression

    • 靶点

      CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where

    • 应用范围

      Western blot (WB)

    • 宿主

      Rabbit

    • 适应物种

      Yersinia pseudotuberculosis

    • 级别

      Serum

    • 供应商

      Agrisera AB

    • 克隆性

      单克隆

    • 保存条件

      Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the c

    • 形态

      Lyophilized

    • 免疫原

      Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII; B1JQV1, NCBI annotated locus tag YPK_41

    • 规格

      50 µl

    The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1.5). To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa. CpxA could be routinely detected from a 20 µL of sample that was derived from 1ml of pelleted bacteria resuspended in 200 µl of 1x loading buffer. Re-use of diluted working strength antibody is not recommended.

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