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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
BSA-conjugated deoxynivalenol
- 形态:
Serum
- 保存条件:
Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing.
- 克隆性:
Polyclonal
- 适应物种:
Fusarium sp.
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
Dot blot (Dot), ELISA (ELISA)
- 靶点:
Anti-DON | Deoxynivalenol, Serum (0,1 ml)
- 抗体英文名:
Anti-DON | Deoxynivalenol, Serum (0,1 ml)
- 抗体名:
Anti-DON | Deoxynivalenol, Serum (0,1 ml)
- 规格:
0.1 ml
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文献和实验Purification Of Anti Peptide Antibody
Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls serum. Wash extensively in TBS after prewashing as indicated in the protocol for coupling
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
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