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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Human Glial cell line-derived neurotrophic factor (GDNF)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
NeuroscienceUniprot ID
P39905Gene Names
GDNFAlternative Name(s)
Astrocyte-derived trophic factor ;ATFLead Time
3-7 business daysOrganism
Homo sapiens (Human)Source
E.coliExpression Region
78-211aaProtein Length
Full Length of Mature ProteinTag Info
N-terminal 6xHis-SUMO-taggedTarget Protein Sequence
SPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCIMW
31.1 kDaPurity
Greater than 90% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验的作用是调控基因表达。例如组蛋白甲基化多导致基因沉默,去甲基化则相反;乙酰化一般是转录激活,去乙酰化则相反。当然,也可在此基础上产生复杂的生物学效应。例如组蛋白去乙酰化酶 HDAC 可影响免疫系统;H3K4me3、H3K9me2 能够调控记忆的形成, 而且 H3K 甲基化与 X 染色体失活、基因组印记和异染色质形成有关;H3 乙酰化通过多种机制调控以来 ATP 的染色质重塑 ,并参与炎症反应;H2A、H2B 泛素化则与 DNA 损害反应有关;而 H3S28 磷酸化与 H3K27 乙酰化可激活转录
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
to a chemoattractant inreal time. Here, we describe using the Dunn chemotaxis chamber to study the responseof murine bone marrow-derived macrophages to colony stimulating factor-1. This is aparticularly useful protocol as it can be adapted to study bone marrow-derived
和执行中非常重要的分子,但某些蛋白质从线粒体内膜腔释放而诱导的凋亡可以不通过caspase产生,称为caspase非依赖性通路。成熟型的丝氨酸蛋白激酶Omi(iV-名HtrA2),在凋亡发生时和Smac一起从线粒体释放到细胞质,一方面灭活1AP,释放caspase,对凋亡复合体起辅助作用,促进凋亡的发生(caspase依赖性通路);另一方面通过其丝氨酸酶活性来激活凋亡,称为caspase非依赖性通路。此外,凋亡诱导因子(apoptosis inducing factor,AIF)位于线粒体的内膜腔内
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