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小鼠Apoc3重组蛋白Apolipoprotein C3Mo

use Apolipoprotein C-III(Apoc3)蛋白
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  • ¥1836
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  • CN
  • CSB-EP001933MO
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Mouse Apolipoprotein C-III (Apoc3)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Mouse Apolipoprotein C-III (Apoc3)

    品名简称:

    Recombinant Mouse Apoc3 protein

    货号:

    CSB-EP001933MO

    规格:

    20μg

    价格:

    1836

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    Apoc3

    Uniprot No.:

    P33622

    别名:

    Apolipoprotein C3

    种属:

    Mus musculus (Mouse)

    蛋白长度:

    Full Length of Mature Protein

    来源:

    E.coli

    分子量:

    24.9 kDa

    表达区域:

    21-99aa

    氨基酸序列:

    EEVEGSLLLGSVQGYMEQASKTVQDALSSVQESDIAVVARGWMDNHFRFLKGYWSKFTDKFTGFWDSNPEDQPTPAIES

    蛋白标签:

    N-terminal 6xHis-SUMO-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assbly and secretion; Extracellular domainly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by rnant receptors.

    功能2:

    Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.

    文献:

    Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications.Puppione D.L., Yam L.M., Bassilian S., Souda P., Castellani L.W., Schumaker V.N., Whitelegge J.P.Biochim. Biophys. Acta 1764:1363-1371(2006)

    研究领域:

    Others

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    相关实验
    • Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by Top-Down MALDI-TOF Mass Spectrometry

      Apolipoprotein C3 (ApoC3) is synthesized in liver so that levels or isoform distributions may constitute indicators of liver pathogenesis. The glycoforms of intact protein ApoC3 in serum or plasma can be readily analyzed by matrix-assisted

    • C3分子

      链在半胱氨酸和谷氨酸残基之间也有一个内硫酯键(Cys-S-CO-Glu)。此环状结构水解后,可形成一个转移性结合点,认为这是C3b由液相结合到固相上的结构条件,也是C3以缓慢的速率水解导致其构象改变出现能与B因子具有亲和力的“变构”C3b的分子基础。当C3转化酶从C3α链N端一个精氨酸-丝氨酸键(第77-78位)外将C3裂解后,可产生一个9kDa的小片段C3a(释放到液相中去),其余部分为C3b。同时,新生的C3bα`链内距N端5kDa的硫酯键断裂,在谷氨酰胺基上出现一个具有转酯作用的高度或多糖

    • C3裂解产物(C3SP)C3a的定量检测

      C3是补体二条激活途径的关键分子,C3活化后裂解为C3a、C3b两个片段,C3a很快被血清羧肽酶N裂解为C3adesarg,C3b被H、I因子灭活为C3bi,经蛋白酶水解为C3c、C3d、C3dg等,检测这些裂解产物即可反映C3的活化程度。C3a的定量检测采用竞争性抑制放射免疫法。其原理为用“I标记C3(I-C3)”与待测样本、兔抗人C3a多克隆抗体一起孵育,待测样本中的C3a(未标记抗原)能竞争性地与抗C3a结合,使C3a复合物(B)增多,而“I-C3”游离出来(F),根据B%来判断待测样本

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