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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Carbamoyl-phosphate synthase [ammonia], mitochondrial (CPS1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Carbamoyl-phosphate synthase [ammonia], mitochondrial (CPS1), partial品名简称:
Recombinant Human CPS1 protein, partial货号:
CSB-YP005913HU规格:
20μg价格:
2208LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CPS1Uniprot No.:
P31327别名:
Carbamoyl-phosphate synthetase I (CPSase I)种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Yeast分子量:
18.4 kDa表达区域:
1354-1500aa氨基酸序列:
GFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQYSAGKAA蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.功能2:
/文献:
"Structural organization of the human carbamyl phosphate synthetase I gene (CPS1) and identification of two novel genetic lesions." Funghini S., Donati M.A., Pasquini E., Zammarchi E., Morrone A. Hum. Mutat. 22:340-341(2003)研究领域:
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文献和实验Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates and Extracts
frequency lock for the spectrometer. Substrate concentrations were 40 mM L-aspartate and 50 mM carbamoyl phosphate, in 0.1 M HEPES buffer (pH 8.0). The reaction was started by adding 100 μl of cell free extract to a total sample volume of 600 μl. To allow
8) 图8-8 UMP的生物合成 (1)合成氨基甲酰磷酸(carbamoyl phosphate):嘧啶合成的第一步是生成氨基甲酰磷酸,由氨基甲酰磷酸合成酶Ⅱ(carbamoyl phosphate synthetase Ⅱ,CPS-Ⅱ)催化CO2与谷氨酰胺的缩合生成。正如氨基酸代谢中所讨论的,氨基甲酰磷酸也是尿素合成的起始原料。但尿素合成中所需氨基甲酰磷酸是在肝线粒体中由CPS-Ⅰ催化合成,以NH3为氮源;而嘧啶合成中的氨基甲酰磷酸在胞液中由CPS�Ⅱ催化生成,利用谷氨酰胺提供氮
也可以从其他物种中去除白蛋白。适用于处理 25 或 50 个样本。Figure 1: 2D analysis of whole human serum before (left) and after(right) treatment with AlbminOUT™. 产品特点: ● 从样本中不到 10 分钟的时间内去除白蛋白 ● 基于白蛋白和 Cibachron 蓝色染料的结合 ● 每个柱子的结合力大于 2 mg 的人白蛋白 ● 从 5-50
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