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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Glutaminase kidney isoform, mitochondrial (GLS), partial, Biotinylated
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥15792.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4464.0 |
| 规格: | 20μg | 产品价格: | ¥2832.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
GLSUniprot No.:
O94925别名:
GLS;K-glutaminase;L-glutamine amidohydrolase种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
54.1 kDa表达区域:
616-669aa氨基酸序列:
KDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL蛋白标签:
N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. ; [Isoform 2]: Lacks catalytic activity.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验GO:0007249:I-kappaB kinase/NF-kappaB cascade PTPLAD1, SNIP1, TLR1, IKBKB, TNIP2 5 0.1317 0.04600 GO:0031980:mitochondrial lumen GLS2, MRPL40, PDPR, FECH, HARS2, TMLHE
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
_004889 Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2 (ATP5J2), mRNA 1513 NM_003769 Homo sapiens splicing factor, arginine/serine-rich 9 (SFRS9), mRNA 1207 NM_003910 Homo sapiens maternal G10 transcript
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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