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大鼠Cps1重组蛋白Carbamoyl-phosphate

synthetase I(CPSase I)Rat Carbamoyl-phosphate synthase [ammonia], mitochondrial(Cps1)蛋白
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  • ¥2328
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  • CN
  • CSB-EP005913RA
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Rat Carbamoyl-phosphate synthase [ammonia], mitochondrial (Cps1), partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Rat Carbamoyl-phosphate synthase [ammonia], mitochondrial (Cps1), partial

    品名简称:

    Recombinant Rat Cps1 protein, partial

    货号:

    CSB-EP005913RA

    规格:

    20μg

    价格:

    2328

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    Cps1

    Uniprot No.:

    P07756

    别名:

    Carbamoyl-phosphate synthetase I (CPSase I)

    种属:

    Rattus norvegicus (Rat)

    蛋白长度:

    partial

    来源:

    E.coli

    分子量:

    23.7 kDa

    表达区域:

    1355-1500aa

    氨基酸序列:

    FKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKARTVDSKSLFHYRQYSAGKAA

    蛋白标签:

    N-terminal 10xHis-tagged and C-terminal Myc-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

    功能2:

    /

    文献:

    "Structural insight on the control of urea synthesis: identification of the binding site for N-acetyl-L-glutamate, the essential allosteric activator of mitochondrial carbamoyl phosphate synthetase." Pekkala S., Martinez A.I., Barcelona B., Gallego J., Bendala E., Yefimenko I., Rubio V., Cervera J. Biochem. J. 424:211-220(2009)

    研究领域:

    Signal Transduction

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    相关实验
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      组织中,分解代谢再产生丙酮酸,后者再接受氨基生成丙氨酸。这一循环途径称为“丙氨酸咸烟茄贰?alanineglucose cycle)。通过此途径,肌肉氨基酸的NH2基,运输到脏脏以NH3或天冬氨酸合成尿素。 饥饿时通过此循环将肌肉组织中氨基酸分解生成的氨及葡萄糖的不完全分解产物丙酮酸,以无毒性的丙氨酸形式转运到肝脏作为糖异生的原料。肝脏异性生成的葡萄糖可被肌肉或其它外周组织利用。 2.氨与谷氨酸在谷氨酰胺合成酶(glutamine synthetase)的催化下生成谷氨

    • Techniques for Measuring the Activity of Carboxylic Acid:CoA Ligase and Acyl‐CoA:Amino Acid N‐Acyltransferase: The Amino Acid Conjugation Pathway

      ]/specific activity of ATP (cpm/pmol) = amount inorganic phosphate formed (pmol)/min/volume enzyme = V . View Image  

    • Measurement of NO and NO Synthase

      of interest Phosphate‐buffered saline (PBS; appendix 2A ; optional) Homogenization buffer (see recipe ) with and without 1 M KCl

    图标技术资料

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    ¥2328