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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Neurofilament heavy polypeptide (Nefh), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Neurofilament heavy polypeptide (Nefh), partial品名简称:
Recombinant Rat Nefh protein, partial货号:
CSB-EP015686RA规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
NefhUniprot No.:
P16884别名:
(NF-H)(200 kDa neurofilament protein)(Neurofilament triplet H protein)种属:
Rattus norvegicus (Rat)蛋白长度:
Partial来源:
E.coli分子量:
44.2 kDa表达区域:
91-410aa氨基酸序列:
ARSEKEQLQALNDRFAGYIDKVRQLEAHNRTLEGEAAALRQQKGRAAMGELYEREVREMRGAVLRLGAARGHVRLEQEHLLEDIAHVRQRLDEEARQREEAEAAARALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKESLERQRSELEDRHQVDMASYQDAIQQLDNELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIG蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NEF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.功能2:
/文献:
Lubec G., Pradeep J.J.P., Afjehi-Sadat L., Kang S.U. Submitted (JUL-2007)研究领域:
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文献和实验大量含于神经元中的外径概为10nm的纤维结构。认为具有保持神经元形态的细胞骨架作用。在此丝的表面,见有毛羽状的构造。一如在轴突内那样神经丝呈平行走向时,两条丝之间也保持着一定的距离。哺乳类动物的神经丝是由分子量20万、16万、6万8千三种多肽构成,这些蛋白质结成一群以极其缓慢的速度(1mm/日)在轴突内移动。神经丝和微管之量比,幼龄动物低,但随着生长、成熟而逐渐增高。另外变性神经元和老化的神经元,其神经丝有异常增加或变形的现象。低等动物的神经丝与哺乳类动物的不同,它是由两种或一种多肽构成
The Role of Calpain in Neurofilament Protein Degradation Associated With Spinal Cord Injury
in glial cells, both calpain isoforms share similar substrate specificities and are inhibited by an endogenous inhibitor, calpastatin (2 ). A broad range of proteins in the CNS, including cytoskeletal and myelin proteins such as neurofilament
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