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大鼠Nefh重组蛋白(NF-H)(200 kDa neuro

filament protein)(Neurofilament triplet H protein)Rat Neurofilament heavy polypeptide(Nefh)蛋白
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  • CSB-EP015686RA
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Rat Neurofilament heavy polypeptide (Nefh), partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Rat Neurofilament heavy polypeptide (Nefh), partial

    品名简称:

    Recombinant Rat Nefh protein, partial

    货号:

    CSB-EP015686RA

    规格:

    20μg

    价格:

    1836

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    Nefh

    Uniprot No.:

    P16884

    别名:

    (NF-H)(200 kDa neurofilament protein)(Neurofilament triplet H protein)

    种属:

    Rattus norvegicus (Rat)

    蛋白长度:

    Partial

    来源:

    E.coli

    分子量:

    44.2 kDa

    表达区域:

    91-410aa

    氨基酸序列:

    ARSEKEQLQALNDRFAGYIDKVRQLEAHNRTLEGEAAALRQQKGRAAMGELYEREVREMRGAVLRLGAARGHVRLEQEHLLEDIAHVRQRLDEEARQREEAEAAARALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKESLERQRSELEDRHQVDMASYQDAIQQLDNELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIG

    蛋白标签:

    N-terminal 10xHis-tagged and C-terminal Myc-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NEF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.

    功能2:

    /

    文献:

    Lubec G., Pradeep J.J.P., Afjehi-Sadat L., Kang S.U. Submitted (JUL-2007)

    研究领域:

    Others

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    相关实验
    • 神经丝 neurofilament

      大量含于神经元中的外径概为10nm的纤维结构。认为具有保持神经元形态的细胞骨架作用。在此丝的表面,见有毛羽状的构造。一如在轴突内那样神经丝呈平行走向时,两条丝之间也保持着一定的距离。哺乳类动物的神经丝是由分子量20万、16万、6万8千三种多肽构成,这些蛋白质结成一群以极其缓慢的速度(1mm/日)在轴突内移动。神经丝和微管之量比,幼龄动物低,但随着生长、成熟而逐渐增高。另外变性神经元和老化的神经元,其神经丝有异常增加或变形的现象。低等动物的神经丝与哺乳类动物的不同,它是由两种或一种多肽构成

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    • Protein production and purification

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    ¥1836