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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Neurofilament heavy polypeptide (NEFH), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Neurofilament heavy polypeptide (NEFH), partial品名简称:
Recombinant Human NEFH protein, partial货号:
CSB-EP015686HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
NEFHUniprot No.:
P12036别名:
200KDA neurofilament protein Neurofilament triplet H protein种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
65.6 kDa表达区域:
1-413aa氨基酸序列:
MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRI蛋白标签:
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.功能2:
Neurofilaments usually contain three intermediate filament proteins文献:
"PKN associates and phosphorylates the head-rod domain of neurofilament protein." Mukai H., Toshimori M., Shibata H., Kitagawa M., Shimakawa M., Miyahara M., Sunakawa H., Ono Y. J. Biol. Chem. 271:9816-9822(1996)研究领域:
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文献和实验个蛋白质点,获取6 张肽质量指纹图谱。通过查询数据库鉴定了9 个蛋白质。通过对过表达脊髓损伤中蛋白质表达谱改变的研究,鉴定出9 个差异表达蛋白质,这些蛋白质包括等。这些差异蛋白质涉及基因为上调蛋白钙蛋白酶Calpain、神经丝蛋白M Neurofilament tripletα12糖蛋白α12Acid2glycoprotein、Oxygen regulatedprotein 1 在脊髓损伤组下调。钙蛋白酶抑制剂Calpain inhibitor、假象蛋白210 Hypothetical
Analysis of mRNA Populations from Single Live and Fixed Cells of the Central Nervous System
. A1, α3 subunit of the GABA‐A receptor; A2, microtubule‐associated protein 2 (MAP2); A3, α‐subunit of calcium/calmodulin kinase II; A4, α‐internexin (courtesy R. Liem); B1, connexin 26 (courtesy D. Paul); B2, connexin 32; B3, human nestin (courtesy U
acidic protein 存在于星形神经胶质细胞和许旺细胞。起支撑作用。 (四)、波形纤维蛋白vimentin 存在于间充质细胞及中胚层来源的细胞中。 (五)、神经纤丝蛋白neurofilament protein 是由三种分子量不同的多肽组成的异聚体,功能是提供弹性使神经纤维易于伸展和防止断裂。二、结构与装配 (一)结构 由螺旋化杆状区,以及两端非螺旋化的球形头(N端)尾(C端)部构成。 杆状区高度保守,由螺旋1和螺旋2构成,每个螺旋区还分为A、B两个亚区。 (二)IF的装配
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