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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Calpain-1 catalytic subunit (Capn1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Calpain-1 catalytic subunit (Capn1)品名简称:
Recombinant Mouse Capn1 protein货号:
CSB-EP004490MO规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Capn1Uniprot No.:
O35350别名:
Calcium-activated neutral proteinase 1 ;CANP 1;Calpain mu-type;Calpain-1 large subunit;Micromolar-calpain ;muCANP种属:
Mus musculus (Mouse)蛋白长度:
Full Length来源:
E.coli分子量:
86.1 kDa表达区域:
1-713aa氨基酸序列:
MTEELITPVYCTGVSAQVQKKRDKELGLGRHENAIKYLGQDYETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKLLDTDLDGVVTFDLFKWLQLTMFA蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal rodeling and signal transduction.功能2:
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.文献:
Poirier C., Poussard S., Faust D.M., Imaizumi-Sherrer T., Weiss M.C., Ducastaing A., Montarras D., Pinset C., Guenet J.-L. Cloning and characterization of the cDNA and gene encoding the mouse mu-calpain large subunit protein.Sahr K.E., Andrabi S., Peters L.L., Chishti A.H. Comprehensive identification of phosphorylation sites in postsynaptic density preparations.Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.Mol. Cell. Proteomics 5:914-922(2006)研究领域:
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文献和实验The Role of Calpain in Neurofilament Protein Degradation Associated With Spinal Cord Injury
Calpain is a calcium-activated neutral proteinase present in all mammalian tissues thus far studied (1 ,2 ). Ubiquitous calpain exists as μ-calpain and m-calpain isoforms which require μM and mM calcium levels for activation
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
receptor cytoplasmic domainI[235]Interleukin-17 (hIL-17)S, 0.35 mg/L, α-MF[236]Intracellular proteinase inhibitor (PI-6)I, 50 mg/L[237]Intrinsic factorS, 40 mg/L, α-MF, functional447Kunitz-type protease inhibitor domain of protease nexin-2/Amyloid b-protein
Studies of the Ubiquitin Proteasome System
, Boston Biochem, or made in house) Wild‐type ubiquitin as reference Additional reagents and equipment for anti‐ubiquitin immunoblotting ( protocol 30 ) Basic Protocol 7: Chelation of Zinc
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