- 询价
- Agrisera
- Sweden
- AS16 3700
- 2026年01月02日
- Dot blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Goat
- Human IgD
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified human IgD,
- 形态：
Immunogen purified goat IgG, in PBS pH 7.2.
- 保存条件：
Stable at 4°C for several years. After reconstitution, the antibody is stable for one week at 4° and for 3-5 years if stored at -20°.
- 克隆性：
Polyclonal
- 适应物种：
Human IgD
- 供应商：
Agrisera
- 宿主：
Goat
- 应用范围：
Dot blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 靶点：
Goat anti-Human IgD (Fc specific), HRP conjugated
- 抗体英文名：
Goat anti-Human IgD (Fc specific), HRP conjugated
- 抗体名：
Goat anti-Human IgD (Fc specific), HRP conjugated
- 规格：
10 mg
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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