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- 文献和实验
- 技术资料
- 保存条件：
见说明书
- 保质期：
见说明书
- 英文名：
Recombinant Mouse Dihydrofolate reductase(Dhfr)
- 库存：
99
- 供应商：
武汉华美生物/CUSABIO
- 规格：
20ug/100ug/1mg
Dhfr蛋白|Recombinant Mouse Dihydrofolate reductase(Dhfr)|大肠杆菌/毕赤酵母/哺乳动物细胞/杆状病毒-昆虫细胞/无细胞 五大表达系统
Uniprot ID：P00375
别名：DhfrDihydrofolate reductase; EC 1.5.1.3
种属：Mus musculus (Mouse)
氨基酸序列：参见官网
微信ID: Cusabio01 电话： 027-87939808
详情请登录：www.cusabio.cn
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文献和实验Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate
Cytogenetic Characterization of Recombinant Cells
The use of mammalian cell systems for recombinant protein production has become the dominant method to produce high-value proteins for therapeutic purposes. Recombinant cell lines are often generated by transfection with specific DNA
Determination of target protein solubility
the cultures for an additional 4–5 hours. Collect a second 1-ml sample (induced control), pellet cells and resuspend in 100 ml 1´SDS-PAGE sample buffer. Freeze until use. 6. Harvest the cells by centrifugation at 4000 ´ g for 20 min. Protein
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