Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
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Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate reductase, to the N-terminus of the target protein, which thereby confers temperature-sensitive degradation at the restrictive temperature. We describe here the application of this technique in the fission yeast,Schizosaccharomyces pombe .
