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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Muscarinic acetylcholine receptor M1 (Chrm1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14844.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3456.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
Chrm1Uniprot No.:
P08482别名:
Chrm1; Chrm-1Muscarinic acetylcholine receptor M1种属:
Rattus norvegicus (Rat)蛋白长度:
Cytoplasmic Domain来源:
Yeast分子量:
19.1 kDa表达区域:
210-366aa氨基酸序列:
RIYRETENRARELAALQGSETPGKGGG_S_SSSSERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDSEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLAKRKTFSLVKEKKAART蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
Identification of a family of muscarinic acetylcholine receptor genes.Bonner T.I., Buckley N.J., Young A.C., Brann M.R.Science 237:527-532(1987)Function:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验The vast majority of receptors are membrane proteins, so solubilization is an essential prerequisite for purification of these receptors. If the receptor can be solubilized without destroying its characteristic ability to bind specific
Overview of Receptor Allosterism
Figure 1.21.1 Allosterism between a receptor and its G protein: the effects of GTP on the competition between [3 H] N ‐methylscopolamine and the agonist, carbachol, at the muscarinic acetylcholine M2 receptor in rat myocardial membranes. Competition
Figure 9.14.6 Saturation data for the binding of [3 H]NMS to the M3 muscarinic receptor. The mean bound cpm is plotted against the amount of added radioligand (radioligand concentration). Specific binding is the difference between total
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