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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Membrane protein MLC1 (MLC1)-VLPs
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Membrane protein MLC1 (MLC1)-VLPs品名简称:
Recombinant Human MLC1 protein-VLPs货号:
CSB-MP613581HU(A5)规格:
20μg价格:
3720LC-MS/Western Blot:
/SDS-PAGE:
CSB-MP613581HU(A5) is detected by Mouse anti-6*His monoclonal antibody.纯度:
The purity information is not available for VLPs proteins.内毒素:
Not test生物活性:
/基因名:
MLC1Uniprot No.:
Q15049别名:
MLC1种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
Mammalian cell分子量:
42.6 kDa表达区域:
1-377aa氨基酸序列:
MTQEPFREELAYDRMPTLERGRQDPASYAPDAKPSDLQLSKRLPPCFSHKTWVFSVLMGSCLLVTSGFSLYLGNVFPAEMDYLRCAAGSCIPSAIVSFTVSRRNANVIPNFQILFVSTFAVTTTCLIWFGCKLVLNPSAININFNLILLLLLELLMAATVIIAARSSEEDCKKKKGSMSDSANILDEVPFPARVLKSYSVVEVIAGISAVLGGIIALNVDDSVSGPHLSVTFFWILVACFPSAIASHVAAECPSKCLVEVLIAISSLTSPLLFTASGYLSFSIMRIVEMFKDYPPAIKPSYDVLLLLLLLVLLLQAGLNTGTAIQCVRFKVSARLQGASWDTQNGPQERLAGEVARSPLKEFDKEKAWRAVVVQMAQ蛋白标签:
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-28 business days注意事项:
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).功能1:
Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx.功能2:
/文献:
/研究领域:
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文献和实验一、简介:1 细胞质膜资料 1895年,Overton从研究细胞透性得出"细胞膜由连续的脂类物质组成"。 1925年 Gorter&Grendel:用脂单分子膜技术测定细胞膜中脂分子的总面积,提出:"细胞膜是由双层脂分子组成"。 1935年 Danielli&Davson:从测定膜的表面张力得出细胞膜的"三明治结构模型",即蛋白质-脂-蛋白质。 1959年 Robertson:用电镜观察生物膜提出
膜运输蛋白(membrane transport protein)
多数代谢上重要的分子和无机离子都不溶于脂,但事实上,它们却可以有效地进出活细胞,而且有精细的控制机制,使其在细胞内外达到适度的浓度梯度,形成一定的膜电位差,这不是简单的扩散,而是通过质膜上特殊的装置——运输蛋白选择性透过的结果。 肌组织中的离子浓度△和稳定的膜电位△ △离子浓度以微当量表示。 运输蛋白根据作用方式分成三类:载体蛋白( car-rier protein)、通道蛋白( channel protein)、离子
Membrane Protein Solubilization
A critical step in any in vitro analysis of membrane proteins is the solubilization of the membrane to extract the protein of interest in an active form to obtain an aqueous solution containing the membrane protein complexed with detergents
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