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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见说明书
- 保质期：
见说明书
- 英文名：
Recombinant Human Myelin basic protein(MBP)
- 库存：
99
- 供应商：
武汉华美生物/CUSABIO
- 规格：
20ug/100ug/1mg
MBP蛋白|Recombinant Human Myelin basic protein(MBP)|大肠杆菌/毕赤酵母/哺乳动物细胞/杆状病毒-昆虫细胞/无细胞 五大表达系统
Uniprot ID：P02686
别名：GDB; Golli MBP; Golli MBP; myelin basic protein; Hemopoietic MBP ; HMBPR; HUGO; MBP; MBP_CAVPO; MBP_人; MGC99675; MLD; Myelin A1 protein; Myelin A1 Protein; basic; Myelin basic protein; Myelin Deficient; Myelin membrane encephalitogenic protein; OTTHUMP00000163776; OTTHUMP00000174387; OTTHUMP00000174388; SHI; Shiverer; SP
种属：Homo sapiens (Human)
氨基酸序列：参见官网
微信ID: Cusabio01 电话： 027-87939808
详情请登录：www.cusabio.cn
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文献和实验Factor Xa Cleavage of MBP-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of factor Xa, temperature and length of incubation must be calibrated
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura Mizoue (refer to the wisdom page for details on the construct). i.Pick a single colony from freshly transformed cells (BL21 (DE3)is the expression host)into 2 ml of LB
Purification of MBP (maLTose-binding proteins) Fused Proteins
. Resuspend pellet in 100 ml buffer for analysis on SDS-PAGE. Elute "unbound" proteins from amylose resin by washing resin with 10 column volumes of 20 mM Tris pH7.4, 200 mM NaCl, 10mM b ME, 1 mM EDTA. Elute bound MBP fusion protein
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