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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
"-20°C/-80°C"
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名:
Customized Bacillus subtilis ylaD Protein (in vitro E.coli)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20ug
Alternative Name(s)
Anti-sigma-ylaC factor ylaDEditorial/Sponsord
EditorialUniprot ID
O07628Gene Names
ylaDOrganism
Bacillus subtilisAASequence
MTCFLVRDLLPLYLEGDCKRETEHVIEEHLKMCSSCRDMYDTMAEPFELESEQAVEEAYL PEEELRFKQRYYGLLIMKAACWFGAAVAMMLIIKLLIExpression Region
1-97aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1378Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动,请以官网信息为准
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文献和实验**产品信息可能有变动,请以官网信息为准
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
Signaling and Sigma-Aldrich). Anti-β-actin (A5441) was from Sigma-Aldrich; horseradish peroxidase-conjugated anti-mouse IgG (#NA931) and anti-rabbit IgG (#NA934) were from GE Healthcare. Detection of antibody reactivity was performed using Immobilon Western
Studies of the Ubiquitin Proteasome System
(see recipe ) 40 mM sodium 2‐oxoglutarate (α‐ketoglutarate, sodium salt; Sigma) 40 mM ascorbic acid (Sigma) Anti‐MBP antibody (Santa Cruz Biotechnology) Boiling water bath
will give the recommended seeding densities. Repeat this every 2-3 days. Key Points If the cell line is a hybridoma or other cell line that produces a substance (e.g. recombinant protein or growth factor) of interest retain the spent media for analysis. 7.Protocol
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