N-(Boc-PEG5)-N-bis(PEG4-acid),

Principles of nucleic acid hybridization

Principles of nucleic acid hybridization 5.2.1. Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within two populations, a complex target population and a comparatively homogeneous probe

核酸探针标记（label of nuclei acid probe ）

的核素：125I 1、常用的放射性核素 1） 32P：产生射线，灵敏度高，分辨率强，是最常用的放射性标记物，但半衰期短（14。3天）：位和位标记。 2）35S：分辨率高、半衰期长（87.1天）。敏感度底 2、放射性标记物的优缺点 优点：灵敏度高：0.01pg 特异性强 缺点：放射性污染 成本高、半衰期短，不能长期保存 二）非放射性标记物 1、优缺点 优点：无放射性污染，成本低、操作简单 缺点：敏感

Synthesis and Use of Pseudopeptides Derived from 1,2,4-Oxadiazole-, 1,3,4-Oxadiazole-, and 1,2,4-Triazole-based Dipeptidomimetics

acids, and the reaction conditions have been chosen so that the enantiopurity is retained during the reaction sequences. Two series of mimetics will be described, one in which the carboxylic acid functionality is directly attached to the heterocyclic