pET-32a(+)

pET-32a(+)
载体类型：大肠杆菌表达载体
载体大小：5900 bp
载体宿主：大肠杆菌（E.coli）
细菌抗性：氨苄青霉素（Amp）
5' 测序引物：S Tag primer
3' 测序引物：T7 terminator primer
载体简介：
The pET-32 series is designed for cloning and high-level expression of peptide sequences fusedwith the 109aa Trx Tag thioredoxin protein . Cloning sites are available for producing fusionproteins also containing cleavable His Tag and S Tag sequences for detection and puri cation.Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression regionof the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented
so that infection with helper phage will produce virions containing single-stranded DNA that corre-sponds to the coding strand. Therefore, single-stranded sequencing should be performed using theT7 terminator primer.

D0084	BL21(DE3)pLysS受体菌	NobleRyder	1ml	100.00
D0083	BL21(DE3)受体菌	NobleRyder	1ml	100.00
D0080	DH5α受体菌	NobleRyder	1ml	100.00
D0085	HB101受体菌	NobleRyder	1ml	100.00
D0098	IPTG 溶液(200mg/ml)	NobleRyder	1ml/5ml	60.00/160.00
D0082	JM109受体菌	NobleRyder	1ml	100.00
D0110	LB培养基	NobleRyder	10L	260.00
D0063	PBR322	NobleRyder	25ug(0.5OD)	100.00
D0064	pET-32a(+)	NobleRyder	25ug(0.5OD)	560.00
D0061	pUC18	NobleRyder	25ug	100.00
D0062	PUC19	NobleRyder	25ug(0.5OD)	100.00
D0081	TOP10受体菌	NobleRyder	1ml	100.00
D0096	X-gal溶液(20mg/ml)	NobleRyder	1ml	40.00
D0099	氨苄青霉素溶液(100mg/ml)	NobleRyder	10ml	100.00