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文献和实验Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
FGFR-AP-FGF Binding Assay, PCR Assays for Knockout Mice, RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of proteins was used in the binding assays... 完整的实验方法请
AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
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