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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
西宝
- 库存:
大量
- 英文名:
BM Purple AP Substrate, precipitating
- 规格:
100 ml
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文献和实验AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
,不能多次检测;在探针拨离前,需要使用二甲基甲酰胺先将膜上沉淀的颜色去除。 其他的底物和抗体 在整个DIG系统中,还有其他的一些底物和多种荧光抗体,例如Fast Red Tablets, BM Purple, HNPP Fluorescent Detection Set 和DAB POD Substrate ;供不同研究的需要。
Human Alkaline Phosphatase staining of transgenic embryo/tissue
. Wash in AP Buffer for 10 minutes. Stain with BM Purple AP Substrate at 4oC for 0.5 to 36 hours (N.B. Incubation at RT will give a quicker result but may result in seepage of staining. Also: NBT/BCIP AP staining has been tried with 9.5dpc embryos
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