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- 2025年07月13日
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文献和实验Microbiology - Serial Dilution
1. An undiluted (stock) solution is termed the 100 dilution. This may be a chemical solution, or a suspension of bacteria or bacteriophage. 2. Typical diluions are 10-fold dilutions; each dilution is 1/10th the concentration of the previous
seconds at 10,000 rpm.Discard flow through and place column back in the collection tube.For each sample to be treated, prepare a DNAse solution by diluting 10 ul of DNAseI （from the kit） into 70 ul of RDD buffer （from the kit） mix by pipetting
gently and incubate on ice for 10 min (for incubations with purified proteins, incubation temperatures may be increased). 3. Add 50 μl of Ca/Mg Solution to the binding reaction at room temperature. 4. Add 1 to 10 μl of DNase I solution (see Hint
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