SsoAdvanced Universal SYBR Gre

逆转录、qPCR 实验还出错？这几点你可能没注意！

%，避免引物内含有互补序列，3』端尽量不要出现含有连续三个以上的 G 或 C 的片段，真核基因设计引物时最好跨内含子哦。 选择合适 ROX 参照染料 各位同学在选购产品、做实验时，首先要弄清自己实验室的仪器对应哪种 ROX（High 还是 Low）！试剂买回来，样也加好了，最后发现不能用在自家仪器上，内心也一定是很崩溃。诺唯赞的染料法荧光定量专用预混液提供各种 ROX 供选择，如果不想这么麻烦，ChamQTM Universal SYBR® qPCR Master Mix （Q711）那是极好

Realtime RT

mixes and Invitrogen mixes with success... -ros- I know you already said you couldn"t afford premixes, but every time I or a co-worker has tried to add Sybr green to a mix the results have been terrible. We are now using Bio-Rad supermix

Realtime RT-PCR problem - no product-Real-Time PCR

I or a co-worker has tried to add Sybr green to a mix the results have been terrible. We are now using Bio-Rad supermix and it is great. I"ll never go back to mixing my own reagents. I also think if you call bio-rad they may be able to send you out a sample