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文献和实验of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
cDNA Synthesis from MOLT-4 Cells
cDNA sample to the center øf the top frit and let it drain into the bed. Collect the effluent into tube 1. 4) Add 100 μl of Ten buffer to the column, and collect the effluent intotube 2.(Note: Let the column drain completely before the addition of new
-HCL PH 6.6, and that is better) Buffer PE 10 mM Tris-HCl pH 7.5 80% ethanol Buffer QBT equilibration buffer 750 mM NaCl 50 mM MOPS pH 7.0 15% isopropanol 0.15% triton X-100 Buffer QC wash buffer
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