iScript gDNA Clear cDNA Synthe

cDNA Synthesis

of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix.   Materials * iScript cDNA Synthesis Kit (BIORAD

cDNA Synthesis from MOLT-4 Cells

First strand of cDNA synthsis 1.Add 2 μl of Not I primer-adapter to a sterile 1.5 ml RNAase free tube, add 4.5 μl mRNA of molt4 cells (~5.2 ug), add 0.5 μl DEPC-H2O. 2. Heat the mixture at 70℃ for 10 min, and quick chill on ice, collect

Miniprep/Qiagen kit

from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB