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文献和实验Estimation of Fat in Milk Samples
Introduction Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessary to know for casein/fat ratio normalization. It is also important
Blocking of unwanted non-specific staining
4- Incubate with skim milk 5 in TBS-T for 10-30 min. (Miller RT et al, Appl. Immunohist & Molecular Morphology, 71(1): 63-65, 1999) NOTE: skim milk may contain weak proteases which will be useful to further unmask antigens, but may reduce immunostaining
Isolation of DNA fragments using glass milk (GENE
and you should start over by spinning out the glass milk and rinsing with NaI solution. 7. Pour off supernatant from the last rinse and respin. Remove the remainder of rinse with a Gilson but don"t allow the pellet to dry. 8. Add 5 ul DDW, vortex
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