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文献和实验Extraction of DNA using DNAzol Reagent
实验概要 Isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. 实验原理 DNAzol® Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA
Genomic DNA Extraction and Barcoding of Endophytic Fungi
, pathogens, and various abiotic stresses. Endophytic fungi have attracted increased interest as potential sources of secondary metabolites with agricultural, industrial, and medicinal use. This chapter provides detailed protocols for isolation of genomic DNA
Extraction of DNA using DNAzol® Reagent
by gently pipetting. Cell Nuclei: Add 1 ml of DNAZOL Reagent to 1-3 × 107 cell nuclei, either in pellet or in suspension (volume To minimize shearing of the DNA molecules, pipet DNA solution using wide-bore pipette tips. Prepare wide bore
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