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文献和实验实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
the secondary antibody at a 1/400 dilution. Apply 150µl and incubate 30 min., RT in a humid chamber. Blot off the antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of ABC-Vector Red complex from the Vector ABC kit (Catalog No. AK
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