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文献和实验A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase I
as described in the Appendix. Two-hundred microliters of the crude extracts was passed through a NICK spin column. Fifty microliters of the eluate was used for GTP cyclohydrolase I assay. After performing the enzyme assay as described in the Methods Section, 30
An Enzymatic Microplate Assay for Testing P-Glycoprotein Substrates and Inhibitors
both an adenosine triphosphatase (ATPase) activity correlated with its drug transport function and a basal ATPase activity in the absence of any drug. The authors have developed an enzymatic test based on modulation of these ATPase activities, which makes
for the analysis of reactive samples. For the anti-HIV part of the assay, confirmation of reactivity should be done with an assay that lacks the p24-antigen detection module and when reactivity persists subsequently by immunoblot. For the p24-antigen
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