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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
rT3 / Reverse Triiodothyronine Antibody
- 库存:
999
- 供应商:
博奥派克
- 规格:
1ml
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
to agarose beads in chromatin samples prior to antibody reaction. (17) Pellet the agarose beads by brief centrifugation (3000-5000 x g (ex. 6600 rpm at Eppendorf table top centrifuge) for 1 min)). (18) Collect supernatants into new tubes. Remove
domesticus x Mus spretus] F1 DNA). In the analysis of the antibody bound (B) and unbound (U) fractions (right panel), the bands representing the maternal and paternal alleles are indicated.MATERIALS Reagents100-bp DNA step ladder (for duplex PCR
Chromatin Immunoprecipitation (ChIP)
cells. Cross-linking is performed by adding formaldehyde to growing cells, and chromatin is prepared, sheared by sonication, and precleared to reduce nonspecific immunoprecipitation. Immunoprecipitation is performed with a specific antibody
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