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文献和实验问:BAF cell的来源是什么?有什么特性和用途呢?答:ATCC上没有你所谓的BAF cell,DSMZ上有两种BAF cell,如下:Cell line:BA-F8Cell type:mouse hybridoma(anti-rat fiber/myosin)Origin:this hybridoma cell line was described to secrete a mouse monoclonal antibody(IgG2b)that reacts with rat type
Chromatin Immunoprecipitation (ChIP)
to be more problematic for monoclonal antibodies or peptide antibodies because these antibody preparations will usually recognize only a single epitope. Therefore, polyclonal antibodies generated against intact native proteins, fusion proteins, or large protein
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
). Day 2 14. If you are not using rabbit polyclonal antibodies (ie. monoclonal antibodies, ect), add 1 ug of an appropriate secondary antibody (rabbit) and incubate for an additional 1 hour. 15. Add
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