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文献和实验Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
and sensitive to changes in both the chemical and mechanical environment. Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins
Interaction of the Glycocalyx with the Actin Cytoskeleton
In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight
Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis
This chapter will focus on responses that the chemoattractant cyclic AMP elicits in the motility system of Dictyostelium . These cells can be permanently transfected to express cytoskeleton-associated proteins tagged with fluorescent proteins
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