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- AnaSpec
- AS-61322
- 2025年12月25日
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文献和实验IN SITU HYBRIDIZATION ON FROZEN SECTIONS
HYBRIDIZATION MIX: for 35S-labeled riboprobe. Assuming that you have used 100µl of prehybridization buffer combine the following: 2.0µl probe per slide (stock solution 300,000 cpm/µl in 1XTE) 1.0 µl tRNA per slide (50 mg/ml stock) Heat 3min, 95°C
IN SITU HYBRIDIZATION ON FROZEN SECTIONS
solution 300,000 cpm/µl in 1XTE) 1.0 µl tRNA per slide (50 mg/ml stock) Heat 3min, 95°C immediately add 17.0µl ice cold rHB2 per slide, vortex, place on ice. (Adjust volumes if you have used less than 100ul for prehybridization). HYBRIDIZATION: Add
. Anatech Lmtd.(616) 964-6450 Eosin: 1X stock. Cat #837. Acid alcohol: 76.6% EtOH, 1/300 v/v conc. HCl. (230 mL EtOH, 70 mL H2 O, 1 mL HCl) Ammonia Solution: 0.084 w/v NH4 OH (1 L H2 O 3 mL 28% w/v NH4 OH stock). Procedure
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