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文献和实验in a low speed centrifuge at 2000 rpm for 5 minutes. If I ligate 100 ng of blunted dephosphorylated vector to 50 ng of a blunt 500 bp PCR in a 30 µl reaction, I'll usually transform 5 ul into cells and plate out the whole tranformation, I normally
Materials: agarose solution in TBE or TAE (generally 0.7-1%) 1X TBE or TAE (same buffer as in agarose) gel loading dye
TRICINE GELS Protocol (For Low MW proteins (
6.6 ul 16 µl 8 µl Add TEMED and APS at the end. Gently swirl the flask to mix, being careful not to generate bubbles. Pipette the solution to a level of 4cm of the top. Add 0.3ml
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