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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Mouse IgM Isotype Control【MS-IGM-5.0 mg】
- 库存:
999
- 供应商:
博奥派克
- 规格:
5.0 mg
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
. Negative control sera: Purified protein (usually mouse IgM or IgG of the same subclass) to match your test antibodies. In flow cytometry, fluorescence is relative. We need a negative control to determine where "positivity" begins. Secondary antibody
Direct Immunofluorescence Labeling
sera: Usually, purified mouse IgM or IgG of the same subclass as the test antibodies and conjugated to the same fluorochrome. One usually purchases them from the same manufacturer to get a similar fluorochrome-to-protein ratio as the test antibodies.
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