Positive Control sgRNA, Human

Selective Silencing of Viral Gene E6 and E7 Expression in HPV-Positive Human Cervical Carcinoma Cells Using Small Interfering RNAs

cancer cells as a model, we demonstrate RNAi for viral oncogenes Cervical cancer is the second most common cancer in women worldwide and is caused by human papillomavirus (HPV). Silencing of HPV E6 and E7 gene expression was achieved using siRNAs

Retroviral Gene Expression Control in Primary Organoid Cultures

: organoid culture; Lgr5?positive stem cells; retrovirus; transgenic animal model; 3R         GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library

Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay

on native agarose gels and detected by ethidium staining. Experimental samples are scord positive for mutations if they contain smaller fragments that are not present in a no-mismatch control.