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啤酒酵母(S. cerevisiae)mRNA的提取

for this protocol.）2. Wash the oligo dT cellulose 3X with 750 l 1X NETS. （Spin down gently between washes and aspirate off the wash.）3. Aspirate off final 1X NETS wash and resuspend the cellulose in 750 l 2X NETS.4. Incubate the RNA at 65 !C for 10 minutes.5. Add

Measurement with the Light Microscope

, the calibration would be 25 µm at 40x, 2.5 µm at 400x, and 1 µm at 1000x. Some stage micrometers are finely divided only at one end. These are particularly useful for determining the diameter of a microscope field. One of the larger divisions is positioned

Chromatin IP (CHIP assay)

lysis buffer (high salt) )      2 x 1 ml CHIP wash buffer      2 x 1 ml TE elute immunoprecipitations: add 75 µl elution buffer incubate for 10 min at 65 °C spin, take supernatant, elute pellet again with 75 µl