Pifithrin-mu/Pifithrin-mu

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth

核酸碱基薄层层析及双波色谱扫描分析准确度验证

液与3μl标准碱基混合液点于同板的不同点上，层析和扫描测定后，选取积分值与标品积分值相差不大的样品量作为测定点样量。取3块薄层板，每板点标品3μl，共3点，样品按预测量点样，亦为3点，照前层析和扫描，分别求出各板中标品和样品相应碱基积分值的平均值，在相应碱基标准曲线上找出标品积分值和15nM 所在的点，从该点推出标准曲线的平行线，即校正曲线，其上由样品积分值对应的X值就是样品的nM数。如果样品和标品积分值接近，也可由样品rim二样品积分值X15"标品积分值，直接算

RNA isolation protocol: cells in culture

DEPC treated water    16 μl 5X first strand buffer    7 μl DTT (0.1 M)    8 μl random primers (concentration = 0.1 μg/μl or a 1/30 dilution of the 3 μg/μl)    8 μl BSA    3 μl dNTPs    1 μl RNAse Out