人小肠上皮细胞

*/ 该细胞为ATCC源细胞，由蒂科生物引进 /*

FHs 74 Int人小肠上皮细胞

ATCCâ Number: CCL-241™

Lot Number: 70019552

 

Name: FHs 74 Int

Description: Small Intestine

Species: Human (Homo sapiens)

Volume/Ampule: Approximately 1 mL

Date Frozen: 09JAN2019

Recovery: A T-25 setup at a seeding density of 1.5 x 104 viable cells/cm2 reaches approximately 15% confluence in 1 day and 90% confluence in 3 days.

Product Format: Cells cryopreserved in the appropriate cryopreservation medium Expiration Date:Not applicable

Storage Conditions: Vapor phase of liquid nitrogen

 

 

Test / Method	Specification	Result
Ampule passage number	Report results	22
Population doubling level (PDL)	Report results	Not applicable
Total cells/ampule (Cell count using Trypan Blue stain method)	Report results	4.4 x 105 total cells/ampule
Post-freeze viability (Cell count using Trypan Blue stain method)	≥ 50.0%	90.8%
Growth properties (Visual observation method)	Adherent	Adherent
Morphology (Visual observation method)	Epithelial-like*	Epithelial-like
Test for mycoplasma contamination Hoechst DNA stain (indirect) method Agar culture (direct) method PCR-based assay	None detected None detected None detected	None detected None detected None detected
Species determination: COI assay (interspecies)	Human	Human

 

 

ATCCâ Number: CCL-241™

Lot Number: 70019552

 

Species determination: STR analysis (intraspecies)	Human (Unique DNA Profile) TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11, 12 D7S820: 9, 10 D16S539: 11, 12 CSF1PO: 10, 11 Amelogenin: X vWA: 16, 18 TPOX: 8	Human (Unique DNA Profile) TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11, 12 D7S820: 9, 10 D16S539: 11, 12 CSF1PO: 10, 11 Amelogenin: X vWA: 16, 18 TPOX: 8
Sterility test (BacT/ALERT 3D) iAST bottle (aerobic) at 32.5°C iNST bottle (anaerobic) at 32.5°C	No growth No growth	No growth No growth
Human pathogenic virus testing (PCR-based assay for HIV, HepB, HPV, EBV, and CMV)	Report results	HIV – None detected HepB – None detected HPV – None detected EBV – None detected CMV – None detected

* Epithelial-like: Any adherent cells of a polygonal shape with clear, sharp boundaries between them.

 

 

Quality Assurance Specialist; Quality Assurance

 

ATCC hereby represents and warrants that the material provided under this certificate is pure and has been subjected to the tests and procedures specified and that the results described, along with any other data provided in this certificate, are true and correct to the best of the company’s knowledge and belief. This certificate does not extend to the growth and/or passage of any living organism or cell line beyond what is supplied within the container received from ATCC.

This product is intended to be used for laboratory research use only. It is not intended for use in humans, animals, or for diagnostics. Appropriate Biosafety Level (BSL) practices should always be used with this material. Refer to the Product Information Sheet for instructions on the correct use of this product.

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