- ￥5820
- Sino Biological
- 北京
- 40150-V05H
- 2026年01月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV Spike/RBD Protein (RBD, mFc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
蛋白名称：Coronavirus spike蛋白, Coronavirus spike protein
蛋白构建：A DNA sequence encoding the receptor binding domain (RBD) of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with the Fc region of mouse IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE.
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV Spike/RBD Protein (RBD, mFc Tag) (40150-V05H), the EC50 of SARS-CoV Spike/RBD Protein (RBD, mFc Tag) is 9-40 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg306
蛋白分子量：The recombinant SARS-CoV Spike/RBD Protein (RBD, mFc Tag) consists of 456 amino acids and predicts a molecular mass of 51.40 kDa.
蛋白NP号：AAX16192.1
蛋白氨基酸序列：Arg306-Phe527
蛋白标签：C-mouse IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Expression, Glycosylation, and Modification of the Spike (S) Glycoprotein of SARS CoV
syndrome (SARS)-coronavirus (CoV) within the host cell, a T7/vaccinia virus-based expression system coupled to immunoprecipitation with anti-S antibodies was used to test and analyze different forms of the S glycoprotein. The state of maturity of the S
Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
the spike protein (S) and the nucleocapsid protein (N), enabling the immunological detection of SARS-CoV by immunofluorescence staining, immunoblot, or an antigen-capture ELISA system. In addition, several S protein-specific antibodies are shown
The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural
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