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文献和实验Antibody Staining of Imaginal Discs
Antibody Staining of Imaginal Discs From DIS- Thom Kaufman's lab 1. Dissect tissues in Tri-PBS, gather in a 1.5 ml tube of Tri-PBS on ice(at least 10 animals can be processed in one tube). Tear larvae
, the discs were washed for 10 minutes each with the following buffers: 50% formamide, 2xSSC (30mM sodium citrate dihydrate, 0.3M sodium chlorine), 0.1% Triton X-100, 0.3% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate) (wash buffer
. doi: 10.1007/s11030-026-11679-z. 7. Chen D, Gu X, Liu J, et al. Notch signaling in the tumor microenvironment: recent advances and targeted therapeutics. Mol Cancer. 2026 Jan 20;25(1):50. doi: 10.1186/s12943-025-02555-9. 8. Chan SW, Ong C, Hong W
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