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文献和实验SECTIONS 4micron paraffin wax sections SOLUTIONS 1. Alcian Blue pH 2.5 Alcian Blue 1g 3% Acetic Acid 100ml 2. 1% Periodic Acid 3. Schiffs Reagent METHOD Take sections
COMBINED ALCIAN BLUE - P.A.S SECTIONS 4micron paraffin wax sections SOLUTIONS 1. Alcian Blue pH 2.5 Alcian Blue 1g 3% Acetic Acid 100ml
Transformation of Electrocompetent E. coli with Blue/White selection
to a bacteria tube. Repeat for additional DNA samples and control DNA. Rotate at 37ºC x30 min. to allow the bacteria to recover. Meanwhile plate LB/Amp plates with IPTG + X-gal if blue/white selection of colonies will be performed
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