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- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
Saturated Ammonium Sulfate Solution
- 保质期：
1年
- 供应商：
上海信裕生物科技有限公司
- 保存条件：
室温保存，一年有效。
- 规格：
500mL
产品简介：
硫酸铵(Ammonium sulfate)分子量为132.14，化学式为(NH4)2·SO4，CAS号为7783-20-2，饱和硫酸铵溶液多用于硫酸铵盐析法沉淀蛋白质。硫酸铵盐析法是分离蛋白质常用的方法之一，其原理是当高浓度的盐加入到蛋白溶液中，铵离子和硫酸根离子同蛋白质竞争与水结合，使蛋白质的溶解性降低而从水溶液中沉淀析出。饱和硫酸铵溶液(SAS)pH为7.0～7.2，操作简单，不易引起蛋白质变性失活，其缺点是所得抗体纯度不高，易受其他蛋白质污染。
试剂保存：室温保存，一年有效。
操作步骤(仅供参考)：按实验具体要求操作。
注意事项：为了您的安全和健康，请穿实验服并戴一次性手套操作。
Ammonium sulphate precipitation is the most commonly used salt ppt because it is highly soluble in water, it stabilizes most proteins in solution (although it may denature some MAbs), and helps reduce the lipid content of the sample.
Ammonium sulphate ppt removes up to 50% of contaminating protein, and therefore reduces the load for subsequent chromatography. Most precipitated proteins retain their activity and native conformation, and can be redissolved easily. Ideal for crude and dilute samples.
TO MAKE A SOLUTION 20% IN AMMONIUM SULFATE (FROM 100% STOCK) ADD 0.25 VOLUMES OF 100% AMMONIUM SULFATE.
Here's an example: Say you have 3 mls of a solution and want to make it 20% ammonium sulfate. The final volume will be 3 mls + x mls where x is the amount of ammonium sulfate you need to add.
Using:
(Final V) x (final C) = (V of concentrate added) x (concentrate C).
Then:
(3 + x) x (20%) = (x) x (100%)
60 + 20x = 100x
60 = 80x
x = 3/4 or 0.75 ml.
so, to bring a 3 ml volume to 20% ammonium sulfate you will need to add 0.75 mls of 100% concentrate.
TO MAKE A SOLUTION 40% IN AMMONIUM SULFATE (FROM 100% STOCK) ADD 0.67 VOLUMES OF 100%% AMMONIUM SULFATE.
(3 + x) x (40%) = (x) x (100%)
120 + 40x = 100x
120 = 60x
x = 2 mls (or 0.67 volumes).
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文献和实验Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
and temperature of the solution. Immunoglobulins precipitate at 40–50% ammonium sulfate saturation depending somewhat on the species and subclass (3 ). The desired saturation is brought about either by addition of solid ammonium sulfate or by addition
Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
as a convenient first step in antibody purification in that, it allows the concentration of the starting material and the precipitation of the desired protein. The principle of ammonium sulfate precipitation lies in “salting out” proteins from the solution
材料和试剂 1. 饱和硫酸铵(saturated ammonium sulfate，SAS)。 2. 透析袋。 3. PBS。 4. 高速低温离心机。 操作步骤 1. 待提取样品经4℃或室温离心13 000r/min(20000×g)，30min，收集上清； 2. 取上清约20ml与等体积生理盐水混合后，于搅拌下缓慢滴加40ml SAS，于4℃沉淀30min以上或过夜，使蛋白充分沉淀。 3. 13 000r/min，10min 4℃离心，弃上清。
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