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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
4℃
- 英文名:
Bio-Rad Laboratories,Inc.
- 库存:
1
- 供应商:
伯乐生命医学(上海)有限公司
- 规格:
5ml
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文献和实验bath for 3 min. 8.Add 100 µl 2x Laemmli sample buffer(or use 75μl of 3X Laemmli sample buffer). 9.Place in boiling water bath for 1 min. 10.Spin briefly in Tomy (at room temp--15 sec,3K),remove liquid with a ml pippetteman tip,and transfer to 1.5 ml
Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
. Resuspend the pellets on ice with a Dounce glass homogenizer and type "A" pestle (as in step 10 of preparation of MT protein above) in ~3x their volume of 1x column buffer containing 0.2 mM GTP. Transfer the resuspended MTs to a 31.5 ml
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