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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温
- 英文名:
Bio-Rad Laboratories,Inc.
- 库存:
5
- 供应商:
伯乐生命医学(上海)有限公司
- 规格:
5ml
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文献和实验Clonality - X Chromosome Inactivation Assay
) Forward and reverse primers, each 20 µM (Human Androgen Receptor Gene (HUMARA) CAG trinucleotide repeat microsatellite primers are described in Allen et al) AmpliTaq Gold, 5 U/ml (Perkin Elmer) a 32P-dCTP, 20 µCi/µl (NEN) Gel-Mix 6 (Life Technologies
Blue Native Gel Electrophoresis
Blue Native Gel Electrophoresis Stock solutions 49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT 3 x Gel buffer 150 mM BisTris-HCl, 1.5 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C
until bromophenyl blue is about 3/4 of the way to the bottom; ~1.5 hrs for a 10% gel). 2. Visualize the oligonucleotides using UV shadowing. Place the gel on saran wrap and place on top of an intensifying screen. Shining short wave UV light from a hand held lamp
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